Isolation of coenzyme B12 from liver.
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چکیده
منابع مشابه
Isolation of Coenzyme B,, from Liver*
The identity of the major cobamide in liver has not been satisfactorily established. Cyanocobalamin (vitamin B12, 5,6dimethylbenzimidazolylcobamide cyanide), which has been obtained in crystalline form with liver as a source material (1, 2), is certainly a very minor component of the cobalamins in liver. Analyses of liver concentrates, both by a paper chromatographicbioautographic method (3) an...
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Vitamin B12 (cobalamin) is an essential coenzyme for nucleic acid synthesis. Animal protein is the major dietary source of vitamin B12. Deficiency of vitamin B12 leads to megaloblastic anemia, degeneration of the brain, spinal cord, peripheral nerves, and abnormalities of epithelial tissues. Two factors are necessary for the cure of megaloblastic anemia: one in food (extrinsic factor) and one i...
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A member of the vitamin B12 group has been identified as the red chromophore of reddish brown enzyme fractions obtained from Clostridium sticklandii. An orange compound which appears to be identical with the coenzyme form of pseudovitamin B12 (or adenine-B12 coenzyme) discovered by Barker et al. (Proe. Natl. Acad. Sci. U. S., 44, 1093, 1958) is obtained if preparations are protected from visibl...
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The in vivo coenzyme A intermediates involved in the metabolic pathway in which vitamin B,t serves as a coenzyme (conversion of methylmalonyl-CoA to succinyl-CoA) were measured in the livers of control and B12-deprived animals. Succinyl-CoA was assayed by the succinate thiokinase arsenolysis of succinyl-CoA coupled with 5’,5’-dithiobis(Znitrobenzoic acid) measurement of the liberated coenzyme A...
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Although cyanocobalamin and hydroxycobalamin are the most commonly encountered supplemental forms of vitamin B12, adenosyland methylcobalamin are the primary forms of vitamin B12 in the human body, and are the metabolically active forms required for B12-dependent enzyme function. Evidence indicates these coenzyme forms of vitamin B12, in addition to having a theoretical advantage over other for...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 236 شماره
صفحات -
تاریخ انتشار 1961